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These conflicting data highlight the importance of directly testing the functional role of WNT5A in prostate cancer progression in appropriate animal models. Received May 17; Accepted Aug All organs were collected and imaged individually in order to detect the presence of metastases. Briefly, cells were plated on coverslips in the presence of Foxy-5, vehicle or a positive control Galiellalactone, see [ 33 ]. Sweden dating stockholm escort service By sexiga trosor bilder äldre kåt kvinna Fest Skara. Mature Mom Lena with young boy. Preliminary unpublished data indicate that the half-life of Foxy-5 in the blood circulation is around 30 minutes in rats.

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Metastasis incidence in multiple organs in mice orthotopically injected with PC3M-Luc2 cells and treated with either vehicle or Foxy

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Int Rev Cell Mol Biol After separation and transfer of the proteins to PVDF membranes, the membranes were blocked and probed with the following antibodies: Cell invasion was then studied as described above. Tissue sections from primary tumors were also analyzed for the presence of cleaved-caspase 3-positive cells and for the presence of CDpositive cells. Primary tumors were dissected and weighed individually; the tumor size was measured using a caliper, and the volume was calculated as previously described [ 36 ].

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